Fig. 6
From: An improved method of constructing degradome library suitable for sequencing using Illumina platform
The modified method can improve the mapping accuracy of the sequencing reads. a, c Alignment of partial rice miR164 and miR167 family precursors (Red letters denote different nucleotides among these miRNA members). The 20-nt tags generated using previous method can be mapped to multiple genes, while the 27-nt tags generated from these genes using the present method can distinguish those differences easily. b, d Tag sequences and frequency obtained from the modified method which contain mature miR164 and miR167 sequence (red letters denote detected nucleotides at mature miRNA end, and the sequence with * indicates this tag derived from miR167 h precursor. Mature miRNA sequences are underlined)