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Table 3 Comparison of main label-based proteomic quantitative techniques

From: Proteomics: a powerful tool to study plant responses to biotic stress

Quantification techniqueLabeling levelMultiplexing capabilityReaction localizationAmino acid residueMSnCharacteristicReferences
15N labelingProtein2-plexIn vivo and in vitroAllMS1Expensive; need metabolically active cells[193]
SILACProtein5-plexIn vivo and in vitroLys, ArgMS1Applicable to active cells; arginine can be converted into proline during cell division[193]
CTAPProtein2-plexIn vivo and in vitroLysMS1Expensive; low multiplexing capability; need stable expression of exogenous enzymes[194]
18O labelingPeptide2-plexIn vitroC-terminalMS1Enzyme-mediated back-exchange of 18O with 16O[195]
ICATProtein/peptide2-plexIn vitroCysMS1Does not support labels without cysteine-containing peptides[196]
ICPLProtein/peptide4-plexIn vitroLysMS1Support clinical samples; need complex computational analysis[197]
TMTPeptide10-plexIn vitroN-terminal, LysMS2Expensive; wide application range[198]
iTRAQPeptide8-plexIn vitroN-terminal, LysMS2High throughout, strong stability; expensive[199]
DiLeuPeptide12-plexIn vitroN-terminal and ε-amino group of the lysine side chainsMS2Wide application range[200]
IPTLPeptide3-plexIn vitroEmploys SA (for N-terminal) and dimethyl (for C-terminal lysine) taggingMS2Wide application range[201]
  1. SILAC stable isotope labeling with amino acids in cell culture, CTAP cell-selective labeling with amino acid precursors, ICAT isotope-coded affinity tag, ICPL isotope-coded protein labeling, TMT tandem mass tag, iTRAQ isobaric tag for relative and absolute quantification, DiLeu N,N-dimethyl leucine, IPTL isobaric peptide termini labeling