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Table 2 New methods and technologies for the identification of PTMs of proteins

From: Proteomics: a powerful tool to study plant responses to biotic stress

PTMs

Method and technology

References

Phosphorylation

Polymer-supported metal-ion-affinity capture

[128]

Functional ligand-binding identification by Tat-based recognition of associating proteins

[129]

Glycosylation

Dendrimer-conjugated benzoboroxole

[130]

Activated ion electron transfer dissociation

[131]

Ubiquitination

Combined fractional diagonal chromatography

[132]

Sumoylation

Replaced SUMO1 and SUMO2 isoforms with a variant and purification

[133]

Acetylation

Peptide prefractionation, immunoaffinity enrichment

[134]

Methylation

Combining SCX, IMAC and H-pH-RPLC

[135]

  1. SCX strong cation exchange, IMAC immobilized metal ion affinity chromatography, H-pH-RPLC high-pH reversed-phase liquid chromatography, SUMO1/2 small ubiquitin-like modifier isoform 1/2