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Table 2 New methods and technologies for the identification of PTMs of proteins

From: Proteomics: a powerful tool to study plant responses to biotic stress

PTMs Method and technology References
Phosphorylation Polymer-supported metal-ion-affinity capture [128]
Functional ligand-binding identification by Tat-based recognition of associating proteins [129]
Glycosylation Dendrimer-conjugated benzoboroxole [130]
Activated ion electron transfer dissociation [131]
Ubiquitination Combined fractional diagonal chromatography [132]
Sumoylation Replaced SUMO1 and SUMO2 isoforms with a variant and purification [133]
Acetylation Peptide prefractionation, immunoaffinity enrichment [134]
Methylation Combining SCX, IMAC and H-pH-RPLC [135]
  1. SCX strong cation exchange, IMAC immobilized metal ion affinity chromatography, H-pH-RPLC high-pH reversed-phase liquid chromatography, SUMO1/2 small ubiquitin-like modifier isoform 1/2