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Table 1 Techniques used for subcellular isolation in plant proteomic studies

From: Proteomics: a powerful tool to study plant responses to biotic stress

Subcellular organelles/proteomeTechniquesPlant/organReferences
Cell wall proteinsExtracted procedure using sequentially CaCl2, EGTA and LiCl-complemented buffersMedicago sativa/Stems[20]
Apoplastic fluidVacuum infiltration-centrifugationGossypium barbadense/Root[21]
Plasma membraneTwo-phase partitioning combined with free-flow electrophoresisArabidopsis/Seedlings[19]
MitochondriaCentrifugation and layered in a Percoll density gradientSolanum tuberosum/Tuber[22]
Centrifugation combined with one-dimensional blue native polyacrylamide gelelectrophoresisArabidopsis/leaf[23]
ChloroplastsHomogenisation in sorbitol-based isolation medium with blender and centrifugationMalus domestica/in vitro material[24]
NucleusFlow cytometric sorting with a mild formaldehyde-based fixationHordeum vulgare/Root[25]
RibosomeAsymmetric flow field-flow fractionationNicotiana benthamiana/transgenic lines[26]