Table 5 Accuracy of UHPLC-QqQ-MS analysis of selected sugar phosphates spiked in Populus leaf extract (mean ± SD, n = 5)
Compound | Added quantity (ng) | Accuracy (%) | Added quantity (ng) | Accuracy (%) |
|---|---|---|---|---|
3-Phosphoglyceric acid | 50 | 88 ± 8 | 150 | 95 ± 6 |
Fructose 1,6-BP | 20 | 79 ± 14 | 60 | 88 ± 12 |
Glucose 1-P | 40 | 102 ± 6 | 120 | 107 ± 9 |
Glucose 6-P | 500 | 105 ± 8 | 150 | 91 ± 10 |
Fructose 6-P | 65 | 92 ± 10 | 195 | 89 ± 8 |
Trehalose 6-P | 85 | 88 ± 9 | 255 | 97 ± 7 |
UDP-glucose | 690 | 85 ± 7 | 2070 | 86 ± 12 |