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Table 2 Lower limit of detection (LLOD), linearity, precision for two standard concentrations (n = 5) and method reproducibility for 5 samples of Populus leaf and wood tissue

From: Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry

Compound

LLOD (pmol)

Linearity range pg; R2

125 pg (%RSD)

2500 pg (%RSD)

Populus leaf (%RSD)

Populus wood (%RSD)

3-Phosphoglyceric acid

0.067

50–10,000; 0.98

6.9

4.1

7.6

16.4

2-Phosphoglyceric acid

0.007

50–10,000; 0.99

8.7

8.1

14.8

22.7

3-Phosphoglyceraldehyde

0.011

50–10,000; 0.99

5.7

3.5

14.7

7.8

Dihydroxyacetone phosphate

0.022

50–20,000; 0.99

4.9

3.6

7.7

6.1

Erythrose-4-P

0.045

50–5000; 0.99

6.4

4.2

10.7

29.8

Ribulose-5-PP

0.091

250–20,000; 0.99

na

4.3

12.6

8.1

Fructose-1,6-PP

0.25

500–20,000; 0.99

na

5.6

14.3

7.7

Xylulose-5-P

0.054

50–5000; 0.99

6.6

7.4

17.9

20.2

Ribulose-5-P

0.054

50–5000; 0.99

4.2

2.1

5.9

8.9

Galactose-1-P

0.029

50–5000; 0.99

7.4

2.5

11.2

4.8

Ribose-5-P

0.026

50–5000; 0.99

4.6

3.1

16.7

7.8

Glucose-1-P

0.024

50–5000; 0.99

1.6

2.2

18.9

11.5

Fructose-6-P

0.012

50–20,000; 0.99

1.5

1.8

7.8

3.5

Glucose-6-P

0.005

50–20,000; 0.99

3.4

2.6

5.7

4.1

Seduheptulose-7-P

0.008

125–20,000; 0.99

2.9

1.7

14.5

9.7

Trehalose-6-P

0.009

50–20,000; 0.99

5.7

1.9

16.2

11.0

Sucrose-6-P

0.011

50–5000; 0.99

6.6

2.9

21.1

17.8

UDP-glucose

0.074

125–10,000; 0.97

6.5

4.7

8.7

8.1

  1. %RSD relative standard deviation in percentage