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Fig. 2 | Plant Methods

Fig. 2

From: A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.

Fig. 2

Transcriptome data and OPLS-DA analysis of CMCs and DDCs from T. wilfordii. a Scattered plot of DEGs analysis between CMCs and DDCs. Genes with red color were up-regulated and genes with green color were down-regulated, blue plot indicates non-DEGs. At an adjusted P < 0.05. b Validation of Cluster 96, Cluster 95, Cluster 71 and Cluster 04 by qRT-PCR. Values are expressed as mean ± SD, n = 3, **P < 0.01 and ***P < 0.001. The expression level of relevant gene of DDC was used as an internal control. c GO classifications of CMC DEGs. Words in red color indicates genes related to vacuoles. d Heatmap of DEGs. The color bar indicates that log10(FPKM + 1) is from high to low. Red indicates up-regulated genes and blue denotes down-regulated genes for CMC or DDC samples shown in triplicate. e Relative expression analysis of TwDXR, TwDXS, TwHMGS, TwHMGR, TwGGPPS, TwFPS and TwIDI genes in the terpenoid biosynthetic pathway. Values are expressed as mean ± SD, n = 3, *P < 0.05 and ***P < 0.001. The expression level of relevant gene of DDC was used as an internal control. f OPLS-DA analysis of CMCs and DDCs. n = 3

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