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Fig. 1 | Plant Methods

Fig. 1

From: A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.

Fig. 1

The isolation and characteristic of CMCs and DDCs from T. wilfordii. a Preparation of T. wilfordii by peeling off cambium, phloem, cortex and epidermal tissue from the xylem and the epidermal tissue side. Cross-section of cambium cell layers (orange arrow) along with phloem tissue. The bottom micrograph shows the xylem. Cells were stained by fast green. White scale bar, 0.25 mm. Black scale bar 10 μm. b A visible natural split of CMCs (red arrow) from DDCs (yellow arrow). Scale bar,1 mm. c Micrographs of CMCs and DDCs. CMCs possess plentiful and small vacuoles, one of which is marked by a black arrow. DDCs only have one large vacuole denoted by a black arrow. Scale bar, 10 μm. d Single CMC and DDC stained with neutral red. The vacuole stained red is indicated by a black arrow. Scale bar, 10 μm. e Effects of zeocin on the death of CMCs and DDCs. Data points represent the mean ± standard deviations (SD), n = 3, *P < 0.05. f Surface morphology of CMC walls observed by AFM. Height image and deflection error image. Scale bar, 4 μm. g Surface morphology of DDC walls observed by AFM. Height image and deflection error image. Scale bar, 4 μm. h The thickness of CMC and DDC walls. Values are expressed as mean ± SD, n = 30, **P < 0.01

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