Fig. 6

Generation and characterization of transgenic events of enset using gfp as reporter gene. a Agro-infected multiple bud explants cultured on selective medium 30 days after transformation, b regeneration of shoots from Agro-infected explants on selective medium, c fully developed transgenic plantlet after 5 cycles of selection for chimera dissociation on medium having 150 mg/l kanamycin, d distinct bud showing expression of gfp gene post 60 days after co-cultivation. e, f Expression of gfp gene in leaf and root tissues excised from transgenic plantlet, g PCR amplification of the nptII gene in different chimera diluted transgenic events. Lane M, 1 Kb plus DNA ladder; Lane 1, pCAMBIA2300-GFP; Lane 2, non-template control; Lane 3, non-transformed control; Lane 4–8, chimera diluted transgenic events 1–5, h RT-PCR analysis of different tissues of the uniformly transformed plantlets using primers specific to nptII gene. Lane M, 100 bp ladder; Lane 1, non-template control; Lane 2, non-transformed control; Lane 3, Leaf of transgenic plantlet; Lane 4, pseudostem of transgenic plantlet; Lane 5, root of transgenic plantlet. i Southern hybridization analysis of transformed plantlets. Genomic DNA was digested with HindIII and probed with a DIG-labelled fragment (780 bp) of nptII gene. Lane M, DIG-labelled Lambda HindIII ladder; Lane WT, non-transformed control DNA; Lane P, pCAMBIA2300-GFP, Scale bar = 1 cm in a–c and 0.5 cm in d–f