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Fig. 5 | Plant Methods

Fig. 5

From: A simple and rapid protocol for the genetic transformation of Ensete ventricosum

Fig. 5

Optimization of different parameters for establishing transformation of enset using multiple buds. a Effect of sonication duration on transient expression of gfp reporter gene as percentage of multiple bud explants showing green fluorescence, b effect of types of cocultivation media on transient expression of gfp gene as percentage of multiple bud explants showing green fluorescence, c effect of Agrobacterium strains on transient expression of gfp gene as percentage of multiple bud explants showing green fluorescence, d effect of resting duration of Agro-infected multiple bud explants surviving on media with 150 mg/l kanamycin. The bars show the mean and standard error for values of expression of gfp gene as percentage of multiple bud explants showing green fluorescence and explant survival on selection media

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