Fig. 1

Regeneration of enset through multiple buds. a Shoot tip, b half split shoot tips used as explants for bud induction, c bud induced (indicated by arrow) from shoot tip explants cultured on the bud induction medium (BIM), d explants showing induced buds (indicated by arrow), e thin slices of excised buds induced on the shoot tips cultured on BIM, f multiple buds induced on explant cultured on the bud multiple medium (BMM), g induction of sprouting of buds on the shoot elongation medium (SEM), h shoots regenerated from multiple buds cultured on SEM having 2.0 mg/l BAP and 0.2% activated charcoal, i complete plantlets on SEM, j enset plantlets undergoing weaning in a humidity chamber in the glasshouse, k potted enset plants growing in the glasshouse. Scale bar = 1 cm for a–d, f–i and 2 mm for e