Fig. 4

Analysis of IAGlc and oxIAGlc synthesized by AtUGTs in a competition assay. a, b IAGlc and oxIAGlc (inset) concentrations measured in supernatant fractions from AtUGT84B1- (a) or AtUGT74D1- (b) producing bacterial cultures. c Levels of IAGlc and oxIAGlc in control GFP-producing bacterial cultures. All the bacterial cultures were incubated with 0.1 mM IAA and/or oxIAA and with UGT cofactor mixture for 6 h at 20 °C and only supernatant fractions were analyzed. Bacterial cultures without IAA and UGT cofactor mixture were used as mock samples. Mean ± SD (n = 3). N.D. not detectable