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Fig. 6 | Plant Methods

Fig. 6

From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

Fig. 6

Applicability of the SR2000/TO-PRO-3 staining technique to different Arabidopsis tissues. Confocal micrographs depict mid-sagittal sections. a–c Inflorescence apex of 5-week-old plant with stage 3 floral meristem (stage according to [77]). a SR2200 staining. b TO-PRO-3 staining. c Merge. Both stains produce clear and specific signals. d–f Main root tip of a 7-day-old seedling. d SR2200 staining. e TO-PRO-3 staining. f Merge. Note TO-PRO-3 signal around the cells and diffuse signal within the cells. Scale bars: 20 μm

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Plant Methods

ISSN: 1746-4811