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Fig. 4 | Plant Methods

Fig. 4

From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

Fig. 4

Nuclear staining of early stage 3 wild-type Arabidopsis ovules with TO-PRO-3 iodide. Confocal micrographs depict mid-sagittal optical sections. a DAPI treatment of an ovule exhibiting a two-nuclear embryo sac (stage 3-II [6]; stage FG2 [74]). Arrows indicate gametophyte nuclei. b TO-PRO-3 iodide treatment of an ovule exhibiting a two-nuclear embryo sac (stage 3-II [6]; stage FG2 [74]). Arrows indicate gametophyte nuclei. c An ovule carrying a four-nuclear embryo sac co-stained with SR2200 and TO-PRO-3 iodide (stage 3-IV [6]; stage FG4 [74]). Note the high-contrast signals labelling cell outlines and nuclei. Scale bars: 10 μm

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