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Fig. 3 | Plant Methods

Fig. 3

From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

Fig. 3

Confocal micrographs depicting mid-sagittal optical sections of fixed early stage 3 wild-type Arabidopsis ovules. a mPS-PI treatment. bd Tissue cleared with ClearSee. b Direct Red 23 staining. c Calcofluor White staining. d SR2200 staining. e Fixed and SR2200-stained ovule with mature embryo sac. Note the weak or absent labelling of embryo sac cells. Arrow indicates weak signal at the synergids. f SR2200 staining in PBS followed by incubation in ClearSee solution. Scale bars: 10 μm

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