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Fig. 2 | Plant Methods

Fig. 2

From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

Fig. 2

Confocal micrographs depicting mid-saggital optical sections of stage 3-V to 3-VI wild-type Arabidopsis ovules carrying fluorescent cell outline markers. a Live ovule expressing the pUBQ::LTi6a:GFP reporter. b Live ovule expressing the pUBQ::LTi6b:GFP reporter. c Live ovule expressing the pSUB::SUB:EGFP reporter. d Fixed ovule expressing the pUBQ::LTi6b:EGFP reporter. The ovule was fixed in 2% formaldehyde for 20 min. Compare to b. e Fixed ovule expressing the pSUB::SUB:EGFP reporter. The ovule was fixed in 2% formaldehyde for 20 min. Compare to c. Scale bars: 10 μm

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Plant Methods

ISSN: 1746-4811