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Fig. 1 | Plant Methods

Fig. 1

From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

Fig. 1

(Adapted from [73])

Micrographs of mature wild-type Arabidopsis ovules. a A mid-sagittal optical section of a stage 3-VI ovule. Clearing by methyl benzoate, Mayer’s hemalum staining, and imaging was done as described earlier [6, 73]. b Scanning electron micrograph of a stage 3-VI/4-I ovule. ap antipodal cells, cc central cell, ec egg cell, et endothelium, fu funiculus, ii inner integument, oi outer integument, syn synergid. Scale bars: 20 μm

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