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Table 1 Effects of promoter driving hygromycin selection and plasmid backbone on transformation efficiency

From: An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)

BackBone Promoter driving the selectable marker gene Number of inoculated IE Number of independent transgenic plants Transformation efficiency (%)
pGGG Act Hyg Intron 150 14 9
pGGG Act Hyg Intron 175 27 15
pGGG Act Hyg Intron 160 40 25
pGGG Act Hyg Intron 100 18 18
pGGG Act Hyg Intron 25 5 20
pGGG Act Hyg Intron 25 6 24
pGGG Act Hyg Intron 80 19 24
pGGG Act Hyg Intron 175 12 7
pAGM Act Hyg Intron 100 15 15
pAGM Act Hyg Intron 100 12 12
pAGM Act Hyg Intron 100 5 5
pAGM Act Hyg Intron 100 14 14
pAGM Act Hyg Intron 100 24 24
pAGM Act Hyg Intron 200 14 7
pAGM Act Hyg Intron 125 21 17
pAGM Act Hyg Intron 150 13 9
pAGM Act Hyg Intron 150 16 11
pAGM Act Hyg Intron 80 18 23
pAGM Act Hyg Intron 150 11 7
pAGM Act Hyg Intron 125 11 9
pAGM Act Hyg Intron 150 15 10
pAGM Act Hyg Intron 150 10 7
pAGM Act Hyg Intron 100 11 11
pBract 35S Hyg Intron 80 4 5
pBract 35S Hyg Intron 150 10 7
pBract 35S Hyg Intron 100 5 5
pBract 35S Hyg Intron 100 5 5
pBract 35S Hyg Intron 100 5 5
pBract 35S-Hyg-Intron 39 2 5
pBract 35S-Hyg-Intron 50 3 6