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Table 1 Effects of promoter driving hygromycin selection and plasmid backbone on transformation efficiency

From: An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)

BackBone

Promoter driving the selectable marker gene

Number of inoculated IE

Number of independent transgenic plants

Transformation efficiency (%)

pGGG

Act Hyg Intron

150

14

9

pGGG

Act Hyg Intron

175

27

15

pGGG

Act Hyg Intron

160

40

25

pGGG

Act Hyg Intron

100

18

18

pGGG

Act Hyg Intron

25

5

20

pGGG

Act Hyg Intron

25

6

24

pGGG

Act Hyg Intron

80

19

24

pGGG

Act Hyg Intron

175

12

7

pAGM

Act Hyg Intron

100

15

15

pAGM

Act Hyg Intron

100

12

12

pAGM

Act Hyg Intron

100

5

5

pAGM

Act Hyg Intron

100

14

14

pAGM

Act Hyg Intron

100

24

24

pAGM

Act Hyg Intron

200

14

7

pAGM

Act Hyg Intron

125

21

17

pAGM

Act Hyg Intron

150

13

9

pAGM

Act Hyg Intron

150

16

11

pAGM

Act Hyg Intron

80

18

23

pAGM

Act Hyg Intron

150

11

7

pAGM

Act Hyg Intron

125

11

9

pAGM

Act Hyg Intron

150

15

10

pAGM

Act Hyg Intron

150

10

7

pAGM

Act Hyg Intron

100

11

11

pBract

35S Hyg Intron

80

4

5

pBract

35S Hyg Intron

150

10

7

pBract

35S Hyg Intron

100

5

5

pBract

35S Hyg Intron

100

5

5

pBract

35S Hyg Intron

100

5

5

pBract

35S-Hyg-Intron

39

2

5

pBract

35S-Hyg-Intron

50

3

6