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Fig. 1 | Plant Methods

Fig. 1

From: An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)

Fig. 1

a–c Selection of wheat spikes and immature embryos at the correct stage, d isolated immature embryo, e immature embryos in Eppendorf tube containing 1 mL WIM, f immature embryos on co-cultivation medium, g immature embryo with the embryonic axis removed before transferring to resting medium, h callus induction on Selection 1 and Selection 2 media, i transformed callus starting to green and produce small shoots, j regenerated shoots with visibly strong roots, k transgenic wheat plant transferred to culture tube showing strong root system in hygromycin containing medium, l transgenic wheat plants before transferring to soil

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Plant Methods

ISSN: 1746-4811