A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Table 3 Luciferase (LUC) measurements obtained in independent experiments after transfection of barley protoplasts

Experiment	Mla1 reference sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sr50 reference sample 7	Sample 8	Sample 9	Sample 10	Sample 11	Sample 12
	Mla1						Sr50
	EV	AVR _a1	AVR_a1-V1	AvrSr50	AvrSr50 _RKQQC	AvrSr50 _QCMJC	EV	AVR _a1	AVR_a1-V1	AvrSr50	AvrSr50 _RKQQC	AvrSr50 _QCMJC
1
LUC measurement	23100	4038	12557	11891	16925	10833	21818	16108	17877	3009	1741	16850
Relative to reference	1	0.17	0.54	0.51	0.73	0.47	1	0.74	0.82	0.14	0.08	0.77
2
LUC measurement	35094	6294	39993	25829	32577	32680	33162	31062	30451	3236	3050	29268
Relative to reference	1	0.18	1.14	0.74	0.93	0.93	1	0.94	0.92	0.10	0.09	0.88
3
LUC measurement	47886	21009	40278	37968	43520	41387	71156	52962	33572	17732	14650	51862
Relative to reference	1	0.44	0.84	0.79	0.91	0.86	1	0.74	0.47	0.25	0.21	0.73
4
LUC measurement	44916	18472	38312	33994	32367	30186	59554	61879	32341	19510	18326	36396
Relative to reference	1	0.41	0.85	0.76	0.72	0.67	1	1.04	0.54	0.33	0.31	0.61

Results are shown for transfections 1–12 into barley mesophyll leaf protoplasts
To compare between independent experiments, values relative to the respective reference samples (reference samples = 1) were calculated

ISSN: 1746-4811