Skip to main content

Table 3 Luciferase (LUC) measurements obtained in independent experiments after transfection of barley protoplasts

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Experiment Mla1 reference sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sr50 reference sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12
Mla1 Sr50
EV AVR a1 AVRa1-V1 AvrSr50 AvrSr50 RKQQC AvrSr50 QCMJC EV AVR a1 AVRa1-V1 AvrSr50 AvrSr50 RKQQC AvrSr50 QCMJC
1
 LUC measurement 23100 4038 12557 11891 16925 10833 21818 16108 17877 3009 1741 16850
 Relative to reference 1 0.17 0.54 0.51 0.73 0.47 1 0.74 0.82 0.14 0.08 0.77
2
 LUC measurement 35094 6294 39993 25829 32577 32680 33162 31062 30451 3236 3050 29268
 Relative to reference 1 0.18 1.14 0.74 0.93 0.93 1 0.94 0.92 0.10 0.09 0.88
3
 LUC measurement 47886 21009 40278 37968 43520 41387 71156 52962 33572 17732 14650 51862
 Relative to reference 1 0.44 0.84 0.79 0.91 0.86 1 0.74 0.47 0.25 0.21 0.73
4
 LUC measurement 44916 18472 38312 33994 32367 30186 59554 61879 32341 19510 18326 36396
 Relative to reference 1 0.41 0.85 0.76 0.72 0.67 1 1.04 0.54 0.33 0.31 0.61
  1. Results are shown for transfections 1–12 into barley mesophyll leaf protoplasts
  2. To compare between independent experiments, values relative to the respective reference samples (reference samples = 1) were calculated