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Table 2 Luciferase (LUC) measurements obtained in independent experiments after transfection of wheat protoplasts

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Experiment Mla1 reference sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sr50 reference sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12
Mla1 Sr50
EV AVR a1 AVR a1 -V1 AvrSr50 AvrSr50 RKQQC AvrSr50 QCMJC EV AVR a1 AVRa1-V1 AvrSr50 AvrSr50 RKQQC AvrSr50 QCMJC
1
 LUC measurement 35605 4001 29003 26349 32102 24521 21382 40966 27441 2042 1438 35195
 Relative to reference 1 0.11 0.81 0.74 0.90 0.69 1 1.92 1.28 0.10 0.07 1.65
2
 LUC measurement 10208 1676 13855 5942 11176 12265 10849 7831 9012 613 439 10,481
 Relative to reference 1 0.16 1.36 0.58 1.09 1.20 1 0.72 0.83 0.06 0.04 0.97
3
 LUC measurement 33215 4487 28457 41008 36279 36824 64846 36707 49537 2126 1974 57030
 Relative to reference 1 0.14 0.86 1.23 1.09 1.11 1 0.57 0.76 0.03 0.03 0.88
4
 LUC measurement 51137 6750 61044 45179 29037 38032 51035 51858 51753 6128 7560 47560
 Relative to reference 1 0.13 1.19 0.88 0.57 0.74 1 1.02 1.01 0.12 0.15 0.93
  1. Results are shown for transfections 1–12 into wheat mesophyll leaf protoplasts
  2. To compare between independent experiments, values relative to the respective reference samples (reference samples = 1) were calculated