Skip to main content

Table 2 Luciferase (LUC) measurements obtained in independent experiments after transfection of wheat protoplasts

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Experiment

Mla1 reference sample 1

Sample 2

Sample 3

Sample 4

Sample 5

Sample 6

Sr50 reference sample 7

Sample 8

Sample 9

Sample 10

Sample 11

Sample 12

Mla1

Sr50

EV

AVR a1

AVR a1 -V1

AvrSr50

AvrSr50 RKQQC

AvrSr50 QCMJC

EV

AVR a1

AVRa1-V1

AvrSr50

AvrSr50 RKQQC

AvrSr50 QCMJC

1

 LUC measurement

35605

4001

29003

26349

32102

24521

21382

40966

27441

2042

1438

35195

 Relative to reference

1

0.11

0.81

0.74

0.90

0.69

1

1.92

1.28

0.10

0.07

1.65

2

 LUC measurement

10208

1676

13855

5942

11176

12265

10849

7831

9012

613

439

10,481

 Relative to reference

1

0.16

1.36

0.58

1.09

1.20

1

0.72

0.83

0.06

0.04

0.97

3

 LUC measurement

33215

4487

28457

41008

36279

36824

64846

36707

49537

2126

1974

57030

 Relative to reference

1

0.14

0.86

1.23

1.09

1.11

1

0.57

0.76

0.03

0.03

0.88

4

 LUC measurement

51137

6750

61044

45179

29037

38032

51035

51858

51753

6128

7560

47560

 Relative to reference

1

0.13

1.19

0.88

0.57

0.74

1

1.02

1.01

0.12

0.15

0.93

  1. Results are shown for transfections 1–12 into wheat mesophyll leaf protoplasts
  2. To compare between independent experiments, values relative to the respective reference samples (reference samples = 1) were calculated
Back to article page

Plant Methods

ISSN: 1746-4811