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Fig. 8 | Plant Methods

Fig. 8

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Fig. 8

Correlation between OD600 and protoplast concentration in protoplast suspensions without cell debris. Isolation of mesophyll leaf protoplasts from barley and wheat should result in suspensions of protoplast free of detectable cell debris (a, protoplast of wheat cv. Fielder). In protoplast suspensions a correlation between the concentration of protoplasts and an optical density measurement allows quantification of protoplast concentration by OD600 measurements in step 15 of this protocol. An OD600 = 0.4 corresponds with 3.5 × 105 protoplasts/ml (b yellow lines), which is the preferred protoplast concentration for subsequent transfection steps

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Plant Methods

ISSN: 1746-4811