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Fig. 6 | Plant Methods

Fig. 6

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Fig. 6

Visual overview of steps for the protoplast isolation from wheat or barley leaves. Step 6: After vacuum infiltration, tube containing leaf tissue is incubated for 3 h at room temperature with 60 rpm shaking in the dark. Step 7: One volume of Wash Buffer is added to 1 volume of Protoplast Isolation Buffer containing leaf tissue. Step 8: Diluted buffer containing leaf tissue is filtered through a pre-moistened 100 µm—nylon cell strainer into a fresh tube. Step 9: Flow through containing protoplasts is centrifuged in round bottom tube at 100 x g for 3 min. Step 10: Supernatant is removed using a pipette. Step 11: Wash buffer is added to protoplast pellet

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Plant Methods

ISSN: 1746-4811