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Fig. 2 | Plant Methods

Fig. 2

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Fig. 2

Results of example setup transfection (Tables 2 and 3) into wheat (a) and barley (b) mesophyll leaf protoplasts based on four biological replicates. Isolated protoplasts were transfected with pUBQ:luciferase and either a pIPKb002 empty vector (EV) control or pIPKb002 vector with cDNAs of AVRa1, AVRa1-V1, AvrSr50WT, AvrSr50RKQQC, AvrSr50QCMJC all lacking respective signal peptides together with either Mla1 or Sr50. Luciferase activity was determined 16 h post transfection as proxy for cell death. * indicate significant differences in luciferase measurements (a, b, non-parametric distribution). Calculated Kruskal–Wallis P values were as follows: a: P = 0.005261, b: P = 0.02896. n.s. not significant (P > 0.05). Experiment was performed four times independently with different plant material used each day and all values (Tables 2 and 3) obtained in the full biological replicates are indicated in turquoise: square; Experiment 1, cross: Experiment 2, triangle: Experiment 3, dot: Experiment 4

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Plant Methods

ISSN: 1746-4811