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Table 4 Number of T1 progeny with indicated genotypes from four transgenic wheat plants that contained Cas9 and sgRNA pairs for TaNFXL1

From: An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

GenotypeNumber of T1 progeny from plant:
NFXL1_1NFXL1_2NFXL1_3NFXL1_4
AXXYY BXXYY DXXYYa39513
Ax2x2y1y1 Bx2x2y2y2 Dx1x2Yy26   
Ax2x2y1y1 Bx2x2y2y2 Dx2x2Yy24   
Ax1x2y1y1 Bx1x1y1y2 Dx2x3Yy1y2 2  
AXx3YY BXx4YY DXXYY  2 
AXx3YY BXXYY DXXYY  2 
AXXYy7 BXXYY DXXYY   5
Other individual unique patterns87152
Incomplete2304
  1. aGenotype code: A, B and D: wheat subgenomes; X: the two alleles for the gene X group in each subgenome, with X as the wild type allele in Fielder and xn for an allele with a distinct editing n; Y and y: alleles for the gene Y group. See Additional file 10 for complete genotyping data