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Table 4 Number of T1 progeny with indicated genotypes from four transgenic wheat plants that contained Cas9 and sgRNA pairs for TaNFXL1

From: An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

Genotype

Number of T1 progeny from plant:

NFXL1_1

NFXL1_2

NFXL1_3

NFXL1_4

AXXYY BXXYY DXXYYa

3

9

5

13

Ax2x2y1y1 Bx2x2y2y2 Dx1x2Yy2

6

   

Ax2x2y1y1 Bx2x2y2y2 Dx2x2Yy2

4

   

Ax1x2y1y1 Bx1x1y1y2 Dx2x3Yy1y2

 

2

  

AXx3YY BXx4YY DXXYY

  

2

 

AXx3YY BXXYY DXXYY

  

2

 

AXXYy7 BXXYY DXXYY

   

5

Other individual unique patterns

8

7

15

2

Incomplete

2

3

0

4

  1. aGenotype code: A, B and D: wheat subgenomes; X: the two alleles for the gene X group in each subgenome, with X as the wild type allele in Fielder and xn for an allele with a distinct editing n; Y and y: alleles for the gene Y group. See Additional file 10 for complete genotyping data
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Plant Methods

ISSN: 1746-4811