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Table 1 Selected sgRNA for TaABCC6, TansLTP9.4 and TaNFXL1

From: An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

sgRNA

Sequence (5′-3′)

ABCC6-sgRNA-1

CACGCCGTCGAGATTACTGG

ABCC6-sgRNA-2

AGTACTCACGGAGATCCAAG

nsLTP9.4-sgRNA-1

GCCGTGCGTGGCGTACGTGA

nsLTP9.4-sgRNA-2

AGTGCTGCTCCGGCGTGCAG

NFXL1-sgRNA-1

TGACTGGCACAACGCAAGGT

NFXL1-sgRNA-2

GATGGAGTTGGTGTGCCGCA

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Plant Methods

ISSN: 1746-4811