Fig. 6

The effect of different washing methods on background and tissue morphology. a Washing temperature: 55 °C, washing solution concentration: 0.2× SSC; washing times: 3 times; 1% (w/v) blocking reagent; blocking time: 45 min; b washing temperature: 58 °C, washing solution concentration: 0.1× SSC; washing times: 5 times; 2% (w/v) blocking reagent; blocking time: 60 min; c washing temperature: 55 °C, washing solution concentration: 0.2× SSC; washing times: 3 times; 2% (W/V) blocking reagent; blocking time: 60 min; the washing process after hybridization was performed with sense probe and antisense probe in different staining tanks. Arrow, hybridized signal was found in the meristem of turnip