Fig. 3

Elicitation of early A. thaliana defence response genes by infiltrating the bacterial peptide flg22: a To provide a strong stress stimulus we used syringe infiltration of either 500 nM flg22 or, as a control, water on a small area of the abaxial side of a leaf (square-1). 1 h after infiltration we dissected 4 squares of a lateral leaf section with square-1 being the infiltration spot, square-2 and square-4 untreated, non-vascular leaf tissue and square-3 as midvein. The figure in b shows the REVIGO [20] treemap of the detected 168 GO-terms grouped under parent terms such as ‘response to organonitrogen compound’ (rose), ‘jasmonic acid metabolism’ (purple), ‘amine catabolism’ (orange) ‘regulation of reactive oxygen species metabolism’ (green), ‘phenol-containing compound metabolism’ (azure blue), ‘respiratory burst’ (blue), etc.. The size of each rectangle relates to the absolute log₁₀ (q-value)—the larger the more significant