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Table 1 The properties of the 5′UTRs used in this study

From: Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

5′UTR

UTR length (bp)

GC content (%)

AAC motifs

Free energy (kcal/mol)a

Deliberately designed structures

5S0

64

34

4

− 4.27

None

5S1

60

30

5

− 1.4

None

5S2

72

32

4

− 10.7

Synthetic CITEb component loop

5S3

93

31

5

− 10.5

Synthetic CITEb and CIREc component loops

5HT

512

41

1

− 110.84

None (viral sequence)

  1. The properties of the 5′UTRs used in this study. a Measured by the mFold secondary structure prediction server [24]; b CITE: 3′Cap-independent translation enhancer; c CIRE: Cap-independent regulatory element