Fig. 5From: Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regionsRelative GFP expression levels for different backbone and UTR combination. GFP was expressed transiently from expression cassettes using different combinations of 5′ and 3′ UTRs and different backbones (see Fig. 2), as indicated on the x-axis. GFP fluorescence was measured in soluble protein extract obtained from agroinfiltrated leaves, and expression is shown relative to pEAQ-HT-GFP (100%, red bar). For each construct, n ≥ 6. Error bars represent 95% confidence intervals; * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001Back to article page