Skip to main content
Fig. 7 | Plant Methods

Fig. 7

From: High throughput procedure utilising chlorophyll fluorescence imaging to phenotype dynamic photosynthesis and photoprotection in leaves under controlled gaseous conditions

Fig. 7

The response of chlorophyll fluorescence parameters (see Table 1 for full descriptions) in the wheat cultivar T. aestivum ‘Pavon76’ to stepwise increases in Photosynthetic Photon Flux Density (PPFD) from 0 to 100% (0–1027 umol m−2 s−1 PPFD) under 20 (white circle) or 200 (black circle) mmol mol−1 O2. After a dark adaptation period of 1 h, maximum PSII efficiency was determined (a—Fv/Fm). From the dark-adapted values of maximal (Fm) and minimal (Fo) fluorescence, the following parameters can be calculated: operating efficiency in the light (b—Fv′/Fm′), PSII quantum yield of PSII (c—Fq′/Fm′), fraction of open PSII reaction centres (d—qP) and non-photochemical quenching (e—NPQ) and. Data are the mean ± SE (n = 4). Asterisks indicate significant differences where ‘***’P < 0.001 ‘**’P < 0.01 ‘*’P < 0.05

Back to article page