Fig. 3

A single Perspex imaging chamber (a) with foam insert and glass lid, gas inlet located below perforated divider plate and sampling outlet located at sample height to monitor gaseous conditions within the chamber. This single chamber can be imaged alone or (b) in combination with three additional chambers to fill the space below the FluorCam camera. The chambers shown in (b) are in-house designed and 3D printed in plastic resin (see Imaging Chamber Design). In this example (c), ~ 120 flag leaf sections were imaged at once in response to a custom protocol. With 1 h dark adaption and a 1 h protocol, it is possible to image up to 480 samples if measuring 8 am–3 pm