Fig. 1

Preparation of C. reflexa/P. zonale infection sites for laser microdissection. a Infection sites were positioned upright in a specimen holder and b 100 µm cross-sections produced using a vibratome. c Cut sections were collected immediately and d stored on ice in tubes with water supplemented with RiboLock RNase Inhibitor before being e transferred to glass slides. After removal of excess liquid using a pipette, f slides with sections were dried in a desiccator at 4 °C before harvesting specific tissue regions by g laser microdissection