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Fig. 3 | Plant Methods

Fig. 3

From: Highly efficient construction of infectious viroid-derived clones

Fig. 3

Infectivity of viroid constructs. a Dot blot of systemic leaves at 21 dpi of ten plants (a1–5/b1–5) agroinfiltrated with HSVd construct (left panel) and ELVd construct (right panel) or and two plants agroinfiltrated with empty vector (Mock a/b). HSVd and ELVd transcripts of plus polarity were used as positive control (+ b). b Northern blot of systemic leaves at 28 dpi of representative cucumber and eggplant plants agroinfiltrated with HSVd construct (1–3 left) and with ELVd construct (1–3 right) respectively. In both case, plants inoculated with empty vectors were used as mock control. Monomeric linear transcript of HSVd and ELVd, respectively were used as positive controls (+). c Figure showing representative symptomatic (cucumber-HSVd) and asymptomatic (eggplant-ELVd) infected plants at 28 dpi

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