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Table 1 Partition coefficients, retention times, diagnostic MRM transitions and optimised instrument settings for the synthesised and studied karrikins

From: Quantification of karrikins in smoke water using ultra-high performance liquid chromatography–tandem mass spectrometry

Compounds

log P

Rt (min)

CV (V)

Quantitation MRM transitions

CE (eV)

Confirmation MRM transitions

CE (eV)

LOD (fmol)

KAR1

− 0.72 ± 0.46

3.62 ± 0.02

35

151 > 123

18

151 > 67

21

1.0

KAR2

− 1.31 ± 0.46

2.09 ± 0.01

30

137 > 81

19

137 > 109

16

0.1

KAR-BR

− 0.96 ± 0.50

3.81 ± 0.02

25

215 > 136

22

215 > 80

27

–

  1. Rt retention time, CV cone voltage, CE collision energy, LOD limit of detection
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Plant Methods

ISSN: 1746-4811