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Fig. 5 | Plant Methods

Fig. 5

From: Heterologous overexpression, purification and functional analysis of plant cellulose synthase from green bamboo

Fig. 5

Size-exclusion profile of BoCesA5 protein during secondary purification. After protease treatment, the protein mixture containing BoCesA5, MBP, and TEV protease was injected into a size-exclusion column (Superdex-200) to isolate BoCesA5 according to molecular weight. The SDS-PAGE results corresponded to the size-exclusion profile through fractions 8–16. The main peak, fraction 8–12, was BoCesA5 protein to be recovered. X-axis: elution volume (mL) in size-exclusion column; Y-axis: absorbance (mAu) at 280 nm

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Plant Methods

ISSN: 1746-4811