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Fig. 2 | Plant Methods

Fig. 2

From: An in vitro protocol for rapidly assessing the effects of antimicrobial compounds on the unculturable bacterial plant pathogen, Candidatus Liberibacter asiaticus

Fig. 2

Time trial for full activation of PMAxx with LED illuminator and PMAxx concentration test. a CLas containing ACP homogenate was aliquoted and treated either with 25 μM PMAxx or water and illuminated for 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5 or 20 min. Samples were immediately taken for DNA extraction after treatment and taken as template for qPCR analysis of CLas 16 s rDNA. Three replicates for each treatment at each time point were run with the values averaged and shown with standard error of the means. The difference between PMAxx treated and untreated samples levels off after 12.5 min. b A group of homogenate aliquots was subjected to the final protocol PMAxx treatment conditions (25 μM PMAxx or water and 15 min illumination). All samples were supplemented with 20 pg of plasmid DNA after PMAxx treatment and qPCR amplified with plasmid specific primers. Shown are the average Ct values from 7 replicates with standard error of the means. Plasmid amplification as measured by Ct was not statistically different in the presence of PMAxx treated samples compared to water, indicating no effect of residual PMAxx

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