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Fig. 4 | Plant Methods

Fig. 4

From: Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology

Fig. 4

a Confocal image (transmission mode) of a trap containing four H2B-mRFP/microtubules-GFP protoplasts. Protoplasts #1 and #2 are in focus and labelled. Scalebar = 40 µm. b Confocal time-lapse imaging of the first division event of the protoplast #2 (purple : H2B-mRFP, green : microtubules-GFP). The GFP channel is in maximum projection mode. Scalebar: 20 µm. c Confocal time-lapse imaging of the cell wall reconstitution around the two protoplasts, using calcofluor-white as a dye. Representation is shown in maximum projection mode. Scalebar: 20 µm. d Mean fluorescence intensity of protoplast 1 over time in the calcofluor-white channel. CV is defined as the ratio of the standard deviation to the mean. The mean intensity increases linearly with time

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