Fig. 1

Representative brightfield microscopy pictures of a suspension of P. patens protoplasts (a) after digestion and purification, and (b) spores (c) Size distributions of protoplasts (Red) and spores (Blue) suspension. A gaussian fit of the size distributions gives a diameter of 32 ± 5 µm (Pooled data of 3 independent measurements with n = 130, 128 and 109 protoplasts) and 32 ± 2 µm respectively (Single measurement of a population of 56 spores). Scale bars: (a) 100 µm, (b) 100 µm