From: Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) | Length (bp) | Efficiency (%) |
---|---|---|---|---|
18S rRNA | TGCTTTGAGCACTCTAATTT | CTTGGGTCGTAAGGGTCGGT | 140 | 90.1 |
Actin(1) | AAAGGCTAACAGGGAGAAAA | GACCACTGGCGTAAAGAGAA | 104 | 107.9 |
Actin(2) | AAACTAGGAAGGAGAAAGGA | AAATAGAGAGCATACAGGGC | 230 | 94.3 |
Actin(3) | GCTTTTCCTTGATGTCTCTC | TCCTGCCATGTATGTTGCTA | 260 | 103.4 |
Actin(4) | GAAAACGGATGGGAGGAGAA | GCTGATAGAATGAGCAAGGA | 238 | 94.9 |
CYP(1) | GTGCCCAAAACAGCAGAAAA | ATCCCCCAGAGTGAAATCCC | 150 | 95.9 |
CYP(2) | GGGTTCAGTAGGAGGGATTT | CTCAGCAGTTTTCGGGGTAA | 140 | 106.3 |
EF1-α(1) | CGAAGGCACAGAAATACCGT | GCTTTTTACCCATCCAAATG | 288 | 108.5 |
EF1-α(2) | AGGTTCTCCACTCAGGCAAC | ACACTCCGCACAATCTCTTC | 260 | 99.4 |
EF1-α(3) | TCACACGGGAAGAGGGAAAG | AGGGTATAGCAGCCAGGAAC | 290 | 96.3 |
EF1-α(4) | GAGCACTCTTTCTCTTTTGA | GAATCTGGTTTAGTCCCTGT | 286 | 97.9 |
EF1-α(5) | AACAGGGGGAAAGGGAAAAC | TGGGGAGGAGAGGAAGGGTA | 220 | 110.4 |
eIF(1) | TCTTCTATGGATGTATGGGG | CGTTCTTGGTGTTGAGGTTT | 114 | 100.9 |
eIF(2) | CAGTGTCTTTTGTTCCGCCT | TTCCTGTGCCCCTACTTGCC | 168 | 99.5 |
eIF(3) | AGGGATGGGAAGAAGGTAAC | ATTCAAAGCATTCAGGGTCA | 192 | 95.3 |
eIF(4) | TCCTTAGCCTGGGATTCTTC | CCTGGTTTCTGAGTTTGCGT | 236 | 99.7 |
eIF(5) | CTCAGAGACTCAGACCCACA | GACCATCCAATGAACCACCT | 218 | 98.6 |
GAPDH | GGTATGAGCAGAATAAAGCA | GGTAATCAATAACAGAACGC | 124 | 96.7 |
histone(1) | AGTCACATACACTGAGCACG | CATAACTAAACGAAGAACCA | 228 | 108.3 |
histone(2) | GGACATTTGATTAGGTTTTG | ATAGATTCTCCCTTTTTTTT | 192 | 95.9 |
histone(3) | TGTCTTTTGATTTGGTGTTT | CTCTCCATTTTCTTTTTGTG | 170 | 92.1 |
histone(4) | GAAAAGTGCTCCGAGACAAC | AACCCCCAAATCCATACAAA | 252 | 96.7 |
histone(5) | TCTCTGGAGCATGTAGAAGC | GGCAAAAACCCATAGACTGT | 226 | 91.3 |
TATA(1) | CATAAAGGAAGGGAGGGAGA | ATGATTACAGCAGCGAAACG | 224 | 97.5 |
TATA(2) | GTTAAATTTCCCATCAGACT | CCAGATACAAAGATAAGCAG | 134 | 99.9 |
TATA(3) | TTCTGCTACATCTGCTCTCT | TTACATTTCTTTCTGCTTCC | 246 | 101.3 |
TUA(1) | AAGGAAGATGCGGCAAATAA | GGAGGGAGTGCGTAGAGAGC | 298 | 106.4 |
TUA(2) | AGAAGGTGTTAAACGAATCA | AGGAAGAGAAAAAGAGGTAG | 180 | 98.1 |
TUB(1) | ATTACTCGGTTACTCCAGCC | AAAGATTCCCCTCTTCAAAA | 102 | 97.4 |
TUB(2) | GGTGGGAGGAGGTGGTGGTA | AGGGAGGGATTCTGAGCCGT | 158 | 93.7 |
UBC(1) | TCTCGGGATAATCTTTGTCA | AGGAACTTCAGGTTGTTGGA | 196 | 105.4 |
UBC(2) | TTTCCATCTGCTCGCTGTTG | GCTCCTTGCTGTGCTCTCGT | 110 | 102.1 |
UBC(3) | TATTCTTTGACTTTTCGCTC | TTCCTGATGCTTATCCCTAC | 262 | 96.9 |
UBC(4) | TGGCTTCCAGACAAATCAAC | GCAACCTACCTCAGCACCTT | 300 | 93.5 |
UBC(5) | TATCAATCCTTCATCCGCCA | CCCTTCCTTATCATCCCTCC | 182 | 99.5 |
UBC(6) | GAAGTTGTCGGAGCTTGGTG | TTTTGGAGGTGAATGAGGGA | 288 | 99.6 |
UBQ(1) | TCGGAAAGGAAAGTCAATTA | CTCACCCACATCAAACACAG | 256 | 105.7 |
UBQ(2) | AATAGACTCAAAATAAGGCG | CCAAGAAAATGGAAGAACTG | 296 | 106.8 |
UBQ(3) | TCAACCAAATAACCCCTGCG | CCTGCCTTGTGGCTCTCACC | 284 | 108.1 |
Cyt P450-like1 | GCTCCAGCCGAACCATACCC | TCTTCCTAAAACTCCGCCAT | 102 | 104.3 |