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Table 1 Primers used in this study

From: Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya

Genes

Forward primer (5′-3′)

Reverse primer (5′-3′)

Length (bp)

Efficiency (%)

18S rRNA

TGCTTTGAGCACTCTAATTT

CTTGGGTCGTAAGGGTCGGT

140

90.1

Actin(1)

AAAGGCTAACAGGGAGAAAA

GACCACTGGCGTAAAGAGAA

104

107.9

Actin(2)

AAACTAGGAAGGAGAAAGGA

AAATAGAGAGCATACAGGGC

230

94.3

Actin(3)

GCTTTTCCTTGATGTCTCTC

TCCTGCCATGTATGTTGCTA

260

103.4

Actin(4)

GAAAACGGATGGGAGGAGAA

GCTGATAGAATGAGCAAGGA

238

94.9

CYP(1)

GTGCCCAAAACAGCAGAAAA

ATCCCCCAGAGTGAAATCCC

150

95.9

CYP(2)

GGGTTCAGTAGGAGGGATTT

CTCAGCAGTTTTCGGGGTAA

140

106.3

EF1-α(1)

CGAAGGCACAGAAATACCGT

GCTTTTTACCCATCCAAATG

288

108.5

EF1-α(2)

AGGTTCTCCACTCAGGCAAC

ACACTCCGCACAATCTCTTC

260

99.4

EF1-α(3)

TCACACGGGAAGAGGGAAAG

AGGGTATAGCAGCCAGGAAC

290

96.3

EF1-α(4)

GAGCACTCTTTCTCTTTTGA

GAATCTGGTTTAGTCCCTGT

286

97.9

EF1-α(5)

AACAGGGGGAAAGGGAAAAC

TGGGGAGGAGAGGAAGGGTA

220

110.4

eIF(1)

TCTTCTATGGATGTATGGGG

CGTTCTTGGTGTTGAGGTTT

114

100.9

eIF(2)

CAGTGTCTTTTGTTCCGCCT

TTCCTGTGCCCCTACTTGCC

168

99.5

eIF(3)

AGGGATGGGAAGAAGGTAAC

ATTCAAAGCATTCAGGGTCA

192

95.3

eIF(4)

TCCTTAGCCTGGGATTCTTC

CCTGGTTTCTGAGTTTGCGT

236

99.7

eIF(5)

CTCAGAGACTCAGACCCACA

GACCATCCAATGAACCACCT

218

98.6

GAPDH

GGTATGAGCAGAATAAAGCA

GGTAATCAATAACAGAACGC

124

96.7

histone(1)

AGTCACATACACTGAGCACG

CATAACTAAACGAAGAACCA

228

108.3

histone(2)

GGACATTTGATTAGGTTTTG

ATAGATTCTCCCTTTTTTTT

192

95.9

histone(3)

TGTCTTTTGATTTGGTGTTT

CTCTCCATTTTCTTTTTGTG

170

92.1

histone(4)

GAAAAGTGCTCCGAGACAAC

AACCCCCAAATCCATACAAA

252

96.7

histone(5)

TCTCTGGAGCATGTAGAAGC

GGCAAAAACCCATAGACTGT

226

91.3

TATA(1)

CATAAAGGAAGGGAGGGAGA

ATGATTACAGCAGCGAAACG

224

97.5

TATA(2)

GTTAAATTTCCCATCAGACT

CCAGATACAAAGATAAGCAG

134

99.9

TATA(3)

TTCTGCTACATCTGCTCTCT

TTACATTTCTTTCTGCTTCC

246

101.3

TUA(1)

AAGGAAGATGCGGCAAATAA

GGAGGGAGTGCGTAGAGAGC

298

106.4

TUA(2)

AGAAGGTGTTAAACGAATCA

AGGAAGAGAAAAAGAGGTAG

180

98.1

TUB(1)

ATTACTCGGTTACTCCAGCC

AAAGATTCCCCTCTTCAAAA

102

97.4

TUB(2)

GGTGGGAGGAGGTGGTGGTA

AGGGAGGGATTCTGAGCCGT

158

93.7

UBC(1)

TCTCGGGATAATCTTTGTCA

AGGAACTTCAGGTTGTTGGA

196

105.4

UBC(2)

TTTCCATCTGCTCGCTGTTG

GCTCCTTGCTGTGCTCTCGT

110

102.1

UBC(3)

TATTCTTTGACTTTTCGCTC

TTCCTGATGCTTATCCCTAC

262

96.9

UBC(4)

TGGCTTCCAGACAAATCAAC

GCAACCTACCTCAGCACCTT

300

93.5

UBC(5)

TATCAATCCTTCATCCGCCA

CCCTTCCTTATCATCCCTCC

182

99.5

UBC(6)

GAAGTTGTCGGAGCTTGGTG

TTTTGGAGGTGAATGAGGGA

288

99.6

UBQ(1)

TCGGAAAGGAAAGTCAATTA

CTCACCCACATCAAACACAG

256

105.7

UBQ(2)

AATAGACTCAAAATAAGGCG

CCAAGAAAATGGAAGAACTG

296

106.8

UBQ(3)

TCAACCAAATAACCCCTGCG

CCTGCCTTGTGGCTCTCACC

284

108.1

Cyt P450-like1

GCTCCAGCCGAACCATACCC

TCTTCCTAAAACTCCGCCAT

102

104.3