Expanding the bioluminescent reporter toolkit for plant science with NanoLUC

Table 1 Model parameters, assuming only decay constants k₃ (NanoLUC) and k₄ (LUC) differ between reporters

Parameter	Value	Units	Source	Process
k ₁	50	cps h⁻¹	Fitted	Translation
k ₂	0.8	h⁻¹	Fitted	BOA decay
k ₃	0.0121	h⁻¹	Experimental	NanoLUC decay
k ₄	0.15	h⁻¹	Fitted	LUC decay
a	0.004	h⁻¹	Fitted	Furimazine decay

Fitting the parameters show that is possible to replicate the signal in Fig. 4d. NanoLUC decay rate was obtained by scaling the experimentally determined rate assuming a Q₁₀ = 2.5 as plate reader experiments were conducted at 21 °C. The NL decay trend could possibly be explained by decay in the furimazine concentration, for which a exponential decay was introduced. Troein et al. [39] estimated a LUC decay constant of ~ 0.183 h⁻¹ in Ostreococcus tauri

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