Parameter
|
Value
|
Units
|
Source
|
Process
|
---|
k
1
|
50
|
cps h−1
|
Fitted
|
Translation
|
k
2
|
0.8
|
h−1
|
Fitted
|
BOA decay
|
k
3
|
0.0121
|
h−1
|
Experimental
|
NanoLUC decay
|
k
4
|
0.15
|
h−1
|
Fitted
|
LUC decay
|
a
|
0.004
|
h−1
|
Fitted
|
Furimazine decay
|
- Fitting the parameters show that is possible to replicate the signal in Fig. 4d. NanoLUC decay rate was obtained by scaling the experimentally determined rate assuming a Q10 = 2.5 as plate reader experiments were conducted at 21 °C. The NL decay trend could possibly be explained by decay in the furimazine concentration, for which a exponential decay was introduced. Troein et al. [39] estimated a LUC decay constant of ~ 0.183 h−1 in Ostreococcus tauri