Fig. 4
From: Expanding the bioluminescent reporter toolkit for plant science with NanoLUC
Dynamics BOA protein using NanoLUC and LUC reporters. a Dynamics in Col-0 CCA1p:LUC BOAp:BOA-NL3F10H using two independent T3 lines, The signal of CCA1p:LUC (triangle) and BOA-NL3F10H (squares) were measured in different wells, the control is shown in red. b Dynamics of BOAp:BOA-LUC in Col-0. Seedlings were grown for 7 days at 100 µmol m2 s−1 white light 12L:12D 21 °C. Then transferred for 2 days into 50 µmol m2 s−1 monochromatic red and blue LEDs 2:1, before quantification started using a Tristar luminometer plate reader every 30 min with 1.5 s of signal integration. The traces are means of 4 replicates. Error bars represent S.E.M. c BOA-NL impacts the period of CCA1p:LUC. X-axis period estimates for CCA1p:LUC time-series from A determined by FTT-NLS from Biodare2. d Inferring species stability by modelling. The experimental data is represented by dotted lines, BOA-NL (blue) and BOA-LUC (yellow). Model traces are represented by solid lines of respective colours. The inference of model parameters was performed with the brightest traces for both BOA-NL and BOA-LUC. The inferred parameters are presented in Table 1