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Fig. 3 | Plant Methods

Fig. 3

From: Expanding the bioluminescent reporter toolkit for plant science with NanoLUC

Fig. 3

Extraction of NL3F10H from tissue of stable transgenic lines. a Comparison of extraction method, (1) manual grinding (mean = 12.42, std = 0.001), (2) treated once with liquid nitrogen and grinded in a Tissue lyser (Qiagen) (mean = 12.95, SD = 0.02). (3) Second round of grinding in Tissue lyser (mean = 13.1, SD = 0.06). (4) Third round of grinding of Tissue lyser (mean = 13.05, std = 0.13). (A) F-value (3,4) = 16.82, p value = 0.009. (B) F-value(2,3) = 1.17, p-value = 0.41. b Testing activity decay after storing tissue at − 80 °C (ln(mean) = 13.58, SD = 0.045) for 2 days compared to liquid nitrogen (LN) (mean = 13.41, SD = 0.01). 10 µl of plant extract mixed with 190 µl of BSII buffer then loaded in a 96-well black plate. Bars represent the mean three biological replicates with error bars representing S.E.M. t-Value [2] = 3.7, p-value = 0.065. All statistical tests performed with ln-transformed data

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