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Fig. 8 | Plant Methods

Fig. 8

From: Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes

Fig. 8

N. benthamiana and tomato plants infected with the PepMV vector carrying the BAR gene. a Schematic representation of the PepBar genome. b and c N. benthamiana and tomato plants inoculated with PepBar or PepMV-Sp13 (wild type virus); photo taken 2 weeks after herbicide treatment. d Agarose gel electrophoresis of RT-PCR products from individual plants to check insert stability in N. benthamiana and tomato plants at 14 d post inoculation (dpi). A minimum of 12 plants were used per treatment, with each treatment replicated a minimum of 3 times. Phenotypes described in the pictures were fully representative of results for the replicates. RdRp: RNA dependent RNA polymerase; TGB1, TGB2 and TGB3: Triple gene block; CP: Coat protein; SGP: CP subgenomic promoter; PepMV wild type virus

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