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Fig. 6 | Plant Methods

Fig. 6

From: Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes

Fig. 6

PepMV vectors expressing fluorescent proteins in tomato plants. a Fluorescence in tomato plants inoculated with PepGFPm2, PepmCherry or PepDsRed at 7 d post inoculation (dpi). b Agarose gel electrophoresis of RT-PCR products from individual plants to check insert stability in tomato at 7 and 12 dpi. c DsRed expression from PepDsRed after the second and third passages at 7 dpi. d Agarose gel electrophoresis of RT-PCR products from individual plants to check insert stability of PepDsRed in tomato passages at 7 dpi. A minimum of 6 plants were used per treatment, with each treatment replicated a minimum of 6 times. Only plants infected with PepDsRed showed systemic fluorescence; all of them in passage 1, 84% and none in passages 2 and 3, respectively.IN: Inoculated leaves; SYS: Systemically infected leaves; FP: fluorescent protein

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Plant Methods

ISSN: 1746-4811