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Fig. 4 | Plant Methods

Fig. 4

From: Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes

Fig. 4

N. benthamiana plants infected with PepMV vectors expressing DsRed and mCherry. a Fluorescence in N. benthamiana plants inoculated with PepDsRed or b PepmCherry visualized under blue light and photographed with an orange filter at 5, 7 and 12 d post inoculation (dpi). c Agarose gel electrophoresis of RT-PCR products from individual plants to check insert stability in vectors infecting N. benthamiana plants at 7 and 12 dpi. A minimum of 6 plants were used per treatment, with each treatment replicated a minimum of 8 times. All infected plants showed systemic fluorescence by 7 dpi. IN: Inoculated leaves; SYS: Systemically infected leaves; FP: Fluorescent protein; PepMV-Sp13, wild type virus

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