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Table 1 Serological tests of A. rhizogenes-induced soybean hairy roots and the performance of back-inoculating soybean with sap from hairy roots, as determined by DAS-ELISA and infectivity assays, respectively

From: Agrobacterium rhizogenes-induced soybean hairy roots versus Soybean mosaic virus (ARISHR-SMV) is an efficient pathosystem for studying soybean–virus interactions

SMV strains ELISA (OD 405 nm)a FCb Infected/inoculated plants
IIc S (%)d
SC3 2.11* 19.18 (+) 18/19 94.7
SC7 2.27* 20.64 (+) 18/18 100
SC8 1.86* 16.91 (+) 14/16 87.5
SC9 2.31* 21.00 (+) 15/15 100
SC10 1.91* 17.36 (+) 21/21 100
SC11 1.75* 15.91 (+) 18/20 90.0
SC13 2.05* 18.64 (+) 17/17 100
SC18 1.92* 17.45 (+) 18/21 85.7
Mock 0.11 1.00 (−) 0/23 0
  1. Each data point represents total number of hairy roots or plants from two replications
  2. *Represents significance difference between treated group and control group of the individual means at 0.05 probability level
  3. aMean absorbance of extract of soybean hairy root at 405 nm
  4. bThe mean OD 405 nm value of each sample and the mean OD 405 nm value of negative control ratio. (+): positive for SMV; (−): negative for SMV
  5. cInfectivity was determined based on the number of infected plants/total number of plants inoculated
  6. dAverage % infection for each SMV strain