Agrobacterium rhizogenes-induced soybean hairy roots versus Soybean mosaic virus (ARISHR-SMV) is an efficient pathosystem for studying soybean–virus interactions

Table 1 Serological tests of A. rhizogenes-induced soybean hairy roots and the performance of back-inoculating soybean with sap from hairy roots, as determined by DAS-ELISA and infectivity assays, respectively

SMV strains	ELISA (OD 405 nm)^a	FC^b	Infected/inoculated plants
SMV strains	ELISA (OD 405 nm)^a	FC^b	II^c	S (%)^d
SC3	2.11*	19.18 (+)	18/19	94.7
SC7	2.27*	20.64 (+)	18/18	100
SC8	1.86*	16.91 (+)	14/16	87.5
SC9	2.31*	21.00 (+)	15/15	100
SC10	1.91*	17.36 (+)	21/21	100
SC11	1.75*	15.91 (+)	18/20	90.0
SC13	2.05*	18.64 (+)	17/17	100
SC18	1.92*	17.45 (+)	18/21	85.7
Mock	0.11	1.00 (−)	0/23	0

Each data point represents total number of hairy roots or plants from two replications
*Represents significance difference between treated group and control group of the individual means at 0.05 probability level
^aMean absorbance of extract of soybean hairy root at 405 nm
^bThe mean OD 405 nm value of each sample and the mean OD 405 nm value of negative control ratio. (+): positive for SMV; (−): negative for SMV
^cInfectivity was determined based on the number of infected plants/total number of plants inoculated
^dAverage % infection for each SMV strain

ISSN: 1746-4811