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Fig. 4 | Plant Methods

Fig. 4

From: Agrobacterium rhizogenes-induced soybean hairy roots versus Soybean mosaic virus (ARISHR-SMV) is an efficient pathosystem for studying soybean–virus interactions

Fig. 4

Verification of P19 and CP transformation events and the effect of P19 and CP on SMV accumulation in soybean hairy roots, respectively. a Soybean calluses were induced by K599 carrying control or destination vector on 10 days under stereo fluorescence microscope. Soybean hairy roots of a cotyledon transformed with control or destination vector are shown on 15 days under white light (b) and long wavelength UV light with a GFP barrier filter (c). d gDNA PCR detection of P19 and CP amplicon in transgenic hairy roots. NT: no template; EV: vector control; +: plasmid; 1#, 2#, 3#: different lines of transgenic hairy roots. e Western blot confirm the presence of P19 and CP protein in hairy roots. U: no plasmid; EV: vector control; P19: P19 plasmid; CP: CP plasmid. f Amount of viral RNA accumulating in hairy roots at 10, 15, and 20 dpi. The error bars indicate standard deviations calculated from three technical replications

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