Fig. 5
Expression analysis of four unigenes encoding enzymes involved in polysaccharide biosynthesis. Relative expression of CL16751.Contig2 (HK), Unigene20408 (scrk), and CL3476.Contig4 and CL3610.Contig2 (sacA) was analyzed by qRT-PCR. The actin gene (CL16529.Contig1) as a reference for normalization of three technical replicates. Root, rhizome, and leaf samples are used as normalizers in each experiment. Blue bars represent qRT-PCR data, and red lines indicate FPKM values. Data represent mean ± standard error of three replicates. The left Y-axis denotes relative expression levels of genes determined by qRT-PCR, and the right Y-axis denotes the FPKM values of RNA-Seq data