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Fig. 5 | Plant Methods

Fig. 5

From: A high-throughput delayed fluorescence method reveals underlying differences in the control of circadian rhythms in Triticum aestivum and Brassica napus

Fig. 5

Increasing temperature causes a shortening of period and effects rhythm robustness. Wheat and Brassica seedlings were entrained for 4 days in L:D at the temperature being assessed before imaging. Each temperature point represents a separate imaging experiment. Period means decrease with increasing temperatures as is shown in a for Brassica (purple circles) or wheat (orange triangles). Error bars represent standard deviation. Box plots show the effect of temperature on RAE for wheat (orange) or Brassica (purple) (b). Period and RAE were estimated by FFT NLLS, BAMP dtr, 24–120 h cut-off. Wheat: 17 °C (N = 17/23); 22 °C (N = 32/32); 27 °C (N = 11/11); 32 °C (N = 15/15). Brassica: 12 °C (N = 24/24); 17 °C (N = 35/35); 22 °C (N = 30/30). An additional preliminary experiment consistent with these observations can be seen in Additional file 1: Supplementary material S13. Significance codes: ***p < 0.001

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